BACKGROUND: This study assessed the bacteriological qualities of surface waters in Afikpo, between April and September 2016.METHODS: Surface water samples were collected from three streams for bacteriological analysis. Bacteria species were isolated using standard microbiological and biochemical techniques. Antibiotic susceptibility study was carried out using Kirby Bauer disc diffusion method.RESULTS: The result of the mean heterotrophic bacteria count from the streams showed that Okpu stream had 209.5CFU/100 mL, Ohino Ngodo 162.5 CFU/100mL, and Ngwogo stream 162.0 CFU/100mL respectively. Out of the twenty-six (26) isolates obtained, E. coli and Staphylococcus species had the highest percentage occurrence (23.1%) respectively.  Klebsiella, Shigella and Enterobacter sp had (11.5%) each, Pseudomonas spp (7.7%), while Salmonella and Streptococcus sp had the least percentage occurrence of (3.8%). The antibiotic susceptibility studies showed that large proportions of isolates were resistant to sulphamethaxoid (SUL), cephalothin (CEP), tetracycline (TET), penicillin G (PEN), oxytetracycline (OXY), cefotaxime (CEF), nalidixic acid (NAL) and cefuroxime sodium (CXM). The most effective antibiotic was azithromycin followed by imipenem. CONCLUSION: The presence of these multi-drug resistant strains in water samples could facilitate transmission of antibiotic resistance. This emphasizes the need for proper treatment of water in the study area.  KEYWORDS: Antibiotic resistance, Bacteria, Afikpo, Surface waters